Figure 6.
Analysis of JA and terpenoid volatiles in WT and irlox2 plants after elicitation. (a) WT plants were wounded and of the wounds immediately treated with either solvent alone, 18:3-Glu (0.17 nmoles), 13-oxo-18:3-Glu or 13-OH-18:3-Glu (see text for a description of the amounts used). Samples were taken after 60 min and JA quantified by LC-MS/MS (n = 3, bars denote ± SE). Different letters denote significant differences (univariate ANOVA F3,3 = 17.9, P < 0.001 followed by a Scheffé post-hoc test, P < 0.05). (b,c) irlox2 and WT plants were wounded and treated with either 18:3-Glu (0.17 nmoles) or 13-oxo-13:2-Glu (see text for a description of the amounts used), the emitted volatiles were trapped for 8 h after 24 h of the treatment and analyzed by GCxGC-ToF. Injection of a β-pinene standard confirmed the structure of the first compound, while the second monoterpene remained unidentified. The MS spectra of the two compounds are shown in Additional file 3. Asterisks indicate significant difference (P < 0.05, Student's t-test).