Figure 1.

Regeneration and transformation of I. walleriana cv. Accent Red. Multiple bud induction 2 weeks after HSCCN explants were cultured on IM2 (A). Multiple shoot production approx. 6 weeks after HSCCN explants were initially cultured on IM2 (B). Transgenic buds expressing GFP produced approx. 3 weeks after selection on IM2 supplemented with 50 mg/l kanamycin and 250 mg/l carbenicillin (C). Transgenic shoots expressing GFP generated approx. 6 weeks after selection on IM2 supplemented with 50 mg/l kanamycin and 250 mg/l carbenicillin and nontransgenic shoots exhibiting dark red autofluorescence (pointed by R) of chlrorophll, indicating no GFP expression (D). Transgenic plantlets expressing GFP approx. 3 weeks after selection on IM4 supplemented with 50 mg/l kanamycin and 250 mg/l carbenicillin (E). GFP segregation in T₁ plants (7 day-old seedlings) (F). A transgenic plant in soil (G). A wild type plant (non-transgenic plant) in soil (H).