Skip to main content
. 2001 Feb;12(2):421–435. doi: 10.1091/mbc.12.2.421

Figure 7.

Figure 7

Fluid-phase endocytosis in rsp5 domain mutants. (A) LY uptake in RSP5 (LHY2066) and rsp5-C2Δ (LHY2232) cells. Cells were grown to early logarithmic phase at 30°C in YPUAD medium and then incubated with LY for 1 h. (B) LY uptake in ww-AXXP mutants. RSP5 (LHY1654), ww1 (LHY1729), ww2 (LHY1734), and ww3 (LHY1735) cells were grown to early logarithmic phase in YPUAD at 24°C, shifted to 30°C for 15 min, and then incubated for 75 min with LY at 30°C. Top panels are fluorescein isothiocyanate images. Bottom panels are differential interference contrast images of the same field of cells. (C) Synthetic lethality of ww1-AXXP and ww3-AXXP mutants with vat2Δ. ww-AXXP and ww-AXXP vat2Δ mutant cells carrying pRSP5(WT)[URA3] were grown on YNB medium containing 5-FOA. The growth of URA3 cells and cells carrying a disruption in VAT2 alone (vat2Δ) are shown for comparison.