Table 3.
Domain | Mutation | Growth at 37°C |
---|---|---|
(wild type) | none | + |
ww1 | W1→F | + |
ww1 | YY→AY | t.s. |
ww1 | YYV→YYA | + |
ww1 | WXXP→AXXP | t.s. |
ww1 | WXXP→AXXA | t.s. |
ww2 | YF→FF | t.s. |
ww2 | WXXP→AXXP | t.s. |
ww2 | WXXP→FXXA | t.s. |
ww3 | YF→AF | t.s. |
ww3 | WXXP→FXXP | + |
ww3 | WXXP→AXXP | t.s. |
ww3 | WXXP→FXXA | t.s. |
rsp5Δ cells carrying the indicated RSP5 plasmids were grown on YPUAD plates at 37°C. t.s. indicates temperature-sensitive slow growth. Resides in bold are those indicated in bold in Figure 5A. W1 indicates the first conserved tryptophan of the WW domain.