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. 2010 Aug 11;10:170. doi: 10.1186/1471-2229-10-170

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Copyright ©2010 Neal et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Gel electrophoresis of PCR-amplified fragments encompassing AOP2 and AOP3 promoter regions and the structural genes. PCR reactions, using genomic DNA from the Col, Pi and Ler as template, were performed with AOP2 and AOP3 gene-specific primers designed to hybridize at flanking positions in promoter regions and CDS regions approximately 300 bp distal from the ATG translation initiation codon. Lanes 1,5 & 9; products obtained from AOP2 promoter and CDS primers, Lanes 2, 6 & 10; products obtained from AOP3 promoter and CDS primers, Lanes 3, 7 & 11; products obtained from AOP2 promoter and AOP3 CDS primers, Lanes 4, 8 & 12; products obtained from AOP3 promoter and AOP2 CDS primers. Lane M contains λ/BstII size markers.