Table 3.
Changes in the relative level of dlps in yellow lupin mitochondria.
| Yellow lupin (Lupinus luteus) | ||||||||
|---|---|---|---|---|---|---|---|---|
| Antibody | K | |||||||
| Organ | Imbibed seeds | Hypocotyls | ||||||
|
Stress treatment |
Size (kDa) | Control | Cold | Heat | Size (kDa) | Control | Cold | Heat |
| 30 | 35.0 | 29.2 | 53.7 | 20 | 44.3 | n.d. | 115.0 | |
| 35 | 42.4 | 107.3 | 62.5 | 25 | 44.2 | 34.5 | 160.5 | |
| 40 | 63.3 | 177.0 | 131.5 | 35 | 54.3 | 120.3 | 85.0 | |
| 45 | 102.1 | 188.8 | 97.7 | 40 | 31.8 | 83.0 | 53.5 | |
| 55 | 171.5 | 183.5 | 144.3 | 45 | n.d. | 44.3 | n.d. | |
| 65/70 | 171.6 | 163.2 | 180.0 | 50/55 | 144.7 | 120.5 | 131.0 | |
| 80 | n.d. | 30.3 | n.d. | 65 | 187.5 | 123.0 | 133.3 | |
| 70 | 175.0 | 179.4 | 179.0 | |||||
| Antibody | DHN24 | |||||||
| Organ | Imbibed seeds | Hypocotyls | ||||||
|
Stress treatment |
Size (kDa) | Control | Cold | Heat | Size (kDa) | Control | Cold | Heat |
| 35 | n.d. | 18.3 | n.d. | 25 | n.d. | 43.1 | n.d. | |
| 40 | 12.5 | 27.2 | 16.8 | 40 | 25.3 | 47.4 | n.d. | |
| 50 | 40.6 | 108.9 | 39.4 | 50 | 121.9 | 148.8 | 79.0 | |
| 55 | 136.8 | 136.1 | 138.2 | 55 | 146.0 | 151.0 | 86.6 | |
| 65 | 87.6 | 123.7 | 78.9 | 65 | 117.8 | 132.3 | 39.7 | |
| 90 | n.d. | 34.1 | 10.3 | 70 | 41.7 | 58.0 | 23.4 | |
Thermostable proteins are denoted in bold. Thermolabile proteins (considered as not resistant for boiling in 15 min.) are underlined. n.d.- not detected or a very low- abundant protein. The thermostability of dlps of the lupin imbibed seed mitochondria was not determined. The results of densitometrical analysis of three independent Western blots were presented as average value in arbitrary units after background subtraction, as the optical density of protein bands per square pixel.