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. 2010 Sep 9;1:114. doi: 10.3389/fphar.2010.00114

Figure 3.

Figure 3

In situ hybridization for RF-amide related peptide (RFRP) and its receptor (OT7T022) in rhesus macaque (Macaca mulatta) testis. RFRP antisense probe labels mRNA in Leydig cells, spermatogonia and spermatocytes (A). An adjacent section incubated with RFRP sense probe (control) is shown in (B). OT7T022 antisense probe labels mRNA in Leydig cells, Sertoli cells, spermatogonia and spermatocytes (C). An adjacent section incubated with OT7T022 sense probe (control) is shown in (D). White arrows, Leydig cells. Gray arrows, spermatogonia. Black arrows, spermatocytes. Black arrowheads, Sertoli cells. All figures to scale, scale bar = 50 μm.