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. Author manuscript; available in PMC: 2011 May 16.
Published in final edited form as: Vaccine. 2010 Mar 10;28(38):6382–6392. doi: 10.1016/j.vaccine.2010.02.097

Table 2.

Antibodies against IsdA and IsdB protect against staphylococcal abscess formation.

Antibodiesa IgG titerb Staphylococcal load in kidneysc Abscess formation
Log10 CFUd Reductione P valuef Abscessesg Reductionh P valuei
Mockj – 6.59 ± 0.15 – – 4.64 ± 1.17 – –
V10 1300 ± 55 6.94 ± 0.34 −0.35 0.2801 2.1 ± 1.1 2.54 0.1432
IsdA 1400 ± 126 5.11 ± 0.79 1.48 0.0181 1.0 ± 0.5 3.64 0.0268
IsdB 1640 ± 320 2.83 ± 0.68 3.76 0.0001 0.86 ± 0.46 3.78 0.0395
IsdBN 875 ± 97 5.54 ± 0.69 1.05 0.0551 1.4 ± 0.54 3.24 0.0382
IsdBC 910 ± 114 3.51 ± 0.99 3.08 0.0001 1.2 ± 0.68 3.44 0.0327
a

Affinity purified antibodies were injected at IgG concentration of 5 mg kg−1 animal weight in 100 μl PBS into the peritoneal cavity of naïve 6-week-old female BALB/c mice.

b

Antibody titers were analyzed by ELISA with purified recombinant antigen (1 μg ml−1) by dilution of serum samples derived via retro-orbital bleeding 24 h following passive immunization.

c

Four days following intravenous challenge with 1 × 107 CFU S. aureus Newman, animals were killed, kidneys excised and tissue homogenate from one randomly chosen kidney spread on agar plates for colony formation and enumeration. Data represent the average and standard error of the means of the log10 CFU from 10 kidneys. Twenty kidneys were analyzed for mock immunized animals injected with 100 μl PBS.

d

Staphylococcal load was enumerated as the log10 of colony forming units (CFU) per gram of kidney tissue homogenate.

e

Reduction in log10 CFU compared to the mock control.

f

Statistical significance was analyzed with the unpaired 2-tailed student's t-test and P values recorded using a cohort of mice (n = 20) for the PBS control group.

g

Average number (and standard error of the means) of abscesses formed in the kidneys of infected mice was enumerated by histopathology of thin-sectioned hematoxylin-eosin stained tissue samples.

h

Reduction in the number of abscesses compared to the mock control.

i

Statistical significance was analyzed with the unpaired 2-tailed student's t-test and P values recorded using a cohort of mice (n = 20) for the PBS control group.

j

Mock immunized animals received an intraperitoneal injection of 100 μl of PBS.