Figure 7.

Quantification of laser speckle imaging using the percent_difference macro (same mouse as Figure 6). This routine subtracts the baseline laser speckle contrast image from the image of interest then divides by the baseline to produce an image based on the percent difference. The image is scaled from 0 to −100% change and is coded with the “fire” lookup table in ImageJ. Not all images can be analyzed in this way owing to movement artifacts resulting in misalignment of the images.