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. 2011 Feb 22;2011:404929. doi: 10.1155/2011/404929

Figure 5.

Figure 5

IFN-γ production and proliferation of spleen cells obtained 4 weeks after TB infection from Ab-treated mice in response to TB antigens (PPD). (a) Spleen cells from each group (n = 5) were pooled and cultured with 20 μg of PPD or TB antigens per mL for 48 h, and then IFN-γ in the culture supernatant was measured. Results are the mean ± S.E.M of triplicate culture. *P < .01 versus corresponding control by Student's t-test. (b) Spleen cells were incubated in the presence of PPD, and BrdU incorporation in the nucleus was measured as O.D. ***(optical density at 340 nm) as an indicator of proliferation. Results are the mean ± S.E.M. of triplicate samples from 5 mice per group. *P < .05 and **P < .01 versus corresponding control by Student's t-test.