Table 1.
Regimen for administration of vehicle and pharmacological agents.
| Treatment | Group | Figure | Administration of vehicle and pharmacological agents | |
|---|---|---|---|---|
| Acquisition trials (Days 1–6) | Retrieval trial (Day 7) | |||
| Control | I (n = 7) | Figures 1A–F, 2(a), and 2(b) | NS (10 mL kg−1 PO) 60 min before + NS (10 mL kg−1 i.p.) 30 min before | NS (10 mL kg−1 PO) 60 min before + NS (10 mL kg−1 i.p.) 30 min before |
| 5% Tween 80 per se | II (n = 7) | Figures 1A–F, 2(a), and 2(b) | Tween 80 (10 mL kg−1 PO) 60 min before + NS (10 mL kg−1 i.p.) 30 min before | Tween 80 (10 mL kg−1 PO) 60 min before + NS (10 mL kg−1 i.p.) 30 min before |
| Scopolamine anterograde | III-V (n = 7/group) | Figures 1(b)–1(d) | Tween 80 (10 mL kg−1 PO) 60 min before + Sco (1 mg kg−1 i.p., 0.5 mg kg−1 i.p., 0.1 mg kg−1 i.p.) 30 min before | Tween 80 (10 mL kg−1 PO) 60 min before + NS (10 mL kg−1 i.p.) 30 min before |
| Scopolamine retrograde | VI–VII (n = 7/group) | Figure 2(b) | Tween 80 (10 mL kg−1 PO) 60 min before + NS (10 mL kg−1 i.p.) 30 min before | Tween 80 (10 mL kg−1 PO) 60 min before + Sco (0.5 mg kg−1 i.p., 0.1 mg kg−1 i.p.) 30 min before |
| Bacopa monniera + Scopolamine 0.5 mganterograde | VIII (n = 7) | Figure 2(b) | bm (120 mg kg−1 PO) 60 min before + Sco (0.5 mg kg−1 i.p.) 30 min before | Tween 80 (10 mL kg−1 PO) 60 min before + NS (10 mL kg−1 i.p.) 30 min before |
| Bacopa monniera + Scopolamine 0.1 mganterograde | IX (n = 7) | Figure 2(b) | BM (120 mg kg−1 PO) 60 min before + Sco (0.1 mg kg−1 i.p.) 30 min before | Tween 80 (10 mL kg−1 PO) 60 min before + NS (10 mL kg−1 i.p.) 30 min before |
| Bacopa monniera + Scopolamine 0.5 mgretrogarde | X (n = 7) | Figures 2E and F | Tween 80 (10 mL kg−1 PO) 60 min before + ns (10 mL kg−1 i.p.) 30 min before | BM (120 mg kg−1 PO) 60 min before + Sco (0.5 mg kg−1 i.p.) 30 min before |
| Bacopa monniera−per se | XI (n = 7) | Figures 1(a), 2(b) | BM (80 mg, 120 mg kg−1 PO) 60 min before | BM (80 mg, 120 mg kg−1 PO) 60 min before |
BM: Bacopa monniera standardized extract suspension in 5% Tween 80, NS: normal saline, n: number of mouse; Sco: scopolamine solution in normal saline; Tween 80: 5% v/v Tween solution in normal saline.