Fig. 1.
MMC-derived soluble factors cause the accumulation of CD11b+Gr1+ cells in FVB mice. FVB mice (3 per group) were injected intradermally with MMC-conditioned medium (CM) or with control medium (RPMI) for 3 consecutive days and killed on the fourth day. a The total percentage of CD11b+Gr1+ cells in the spleens, bone marrow, and blood of medium-treated (RPMI) or CM-treated (CM) mice as measure by flow cytometry. b Multiplex array analysis of MMC-derived supernatant after a 24-h culture shows substantial levels of GM-CSF, VEGF, and MCP-1. Data are averages of duplicate samples