Fig 2.

MMC-derived soluble factors and GM-CSF can generate CD11b+Gr1+ cells from the CD11b-Gr1-progenitor cells, and can maintain existing CD11b+Gr1+ cells. Bone marrow cells from naïve FVBN202 mice were stained and sorted into two populations: CD11b+Gr1+ and CD11b-Gr1-cells. a Flow cytometry plots of the purity of CD11b+Gr1+ and CD11b-Gr1- populations after sorting. Data are representative of three experiments. b Representative flow cytometry plots showing the percentage of CD11b+Gr1+ cells 6 days after culture of sorted CD11b-Gr1-cells (left) and averages of 3–4 experiments (right). c Representative flow cytometry plots showing the percentage of CD11b+Gr1+ cells remaining on day 6 after culture of sorted CD11b+Gr1+ cells (left) and averaged data from 3–4 experiments (right). (d) Representative flow cytometry staining for CD11b and Gr1 from naïve FVBN202 BM cells on day 0 (left) and after 6 days of culture with 100 ng/ml of GM-CSF. Data are representative of 3–5 separate experiments. e Representative data from duplicate experiments showing BrdU incorporation in gated CD4+ and CD8+ T cells derived from FVB mice and cultured for 72 h in the absence (top panel) or presence of CD11b+Gr1+ cells (bottom panel) generated from culture of unfractionated bone marrow cells (d) with GM-CSF