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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Breast Cancer Res Treat. 2009 Nov 8;123(1):39–49. doi: 10.1007/s10549-009-0622-8

Fig. 3.

Fig. 3

MMC-derived soluble factors and GM-CSF can protect newly derived and existing CD11b+Gr1+ cells from apoptosis. Sorted populations of CD11b+Gr1+ or CD11b-Gr1-cells were cultured for 6 days with medium, MMC, or GM-CSF and stained on day 6 for CD11b, Gr1, and Annexin V. a Representative flow cytometry plots of duplicate experiments showing the expression of Annexin V on sorted CD11b+Gr1+ cells (left) or sorted CD11b-Gr1- cells (right) just after sorting. b Representative flow cytometry plots of CD11b+Gr1+ cells after 6 days of culture of sorted CD11b-Gr1- cells (boxed region) that are Annexin V positive. Bar graph shows the average percentage of CD11b+Gr1+AnnexinV+ cells from 3–4 experiments. c Representative flow cytometry plots of CD11b+Gr1+ after 6 days of culture of sorted CD11b+Gr1+ cells (boxed region) that are Annexin V positive. Bar graph depicts the averages of 3–4 experiments