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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Breast Cancer Res Treat. 2009 Nov 8;123(1):39–49. doi: 10.1007/s10549-009-0622-8

Fig. 5.

Fig. 5

MMC-derived supernatant and GM-CSF cause the generation of suppressive CD11b+Ly6G−Ly6C+ MDSC from CD11b−Gr1− precursor cells. a Sorted CD11b+Gr1+ cells from bone marrow (BM) of tumor-free FVBN202 mice (n = 3) were analyzed on day 0 for the expression of Ly6G and Ly6C by flow cytometry. CD11b−Gr1− cells sorted from these mice and cultured with GM-CSF were analyzed on day 6 for the expression of Ly6G and Ly6C by flow cytometry. b Representative data from duplicate experiments showing BrdU incorporation in gated CD4+ and CD8+ T cells 72 h after culture in the absence (top panel) or presence of cells (bottom panel) that were generated from a 6-day culture of CD11b−Gr1− progenitor cells with GM-CSF. c Gated neu positive MMC were analyzed for Annexin V and PI after a 48 h culture with neu-specific T cells. d Representative data from duplicate experiments showing BrdU incorporation in gated CD4+ and CD8+ T cells 72 h after culture in the absence (top panel) or presence of CD11b+Gr1+ cells derived from tumor-free mice and used at 1:1 (middle panel) or 1:2 (bottom panel) rations