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PS content of the bilayer controls C2AB binding density and particle size. Supported lipid bilayers with bound C2AB (initial concentration of 1 µM) were produced from liposomes composed of DOPC and PS at ratios of 3:1 (A and B) and 1:1 (C and D). Typical images are shown in panels A and C. Note the presence of large sheetlike structures attached to the bilayer in panel C. (B and D) Frequency distributions of particle sizes. Peak molecular volumes are indicated.
