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. 2010 Oct 25;2011:238208. doi: 10.1093/ecam/nep021

Table 1.

Oligonucleotide primer sets used for semi-quantitative RT-PCR.

RNA target Primer sets Oligonucleotide sequence Annealing temperature (°C) Product size (bp) Accession no.
INF-γ F 5′-AGCTGACGGTGGACCTATTA-3′ 56 259 NM_205149
R 5′-GGCTTTGCGCTGGATTCTC-3′
IL-2 F 5′-CTGGGACCACTGTATGCTCT-3′ 55 256 AF017645
R 5′-CACCAGTGGGAAACAGTATC-3′
IL-4 F 5′-ACCCAGGGCATCCAGAAG-3′ 55 258 AJ621735
R 5′-CAGTGCCGGCAAGAAGTT-3′
IL-12 F 5′-AGACTCCAATGGGCAAATGA-3′ 56 244 NM_213571
R 5′-CTCTTCGGCAAATGGACAGT-3′
GAPDH F 5′-GGTGGTGCTAAGCGTGTTAT-3′ 56 264
R 5′-ACCTCTGTCATCTCTCCACA-3′