(A) Cells of the indicated genotype were grown asynchronously at room temperature and examined by immunofluorescence microscopy, staining for tubulin and DAPI. Cells were scored for cell cycle stage, defining a metaphase cell as large-budded with a single DNA mass and short bipolar spindles. Over 200 cells were counted in two independent experiments; error bars represent the standard error of the mean (SEM).
(B) Strains containing a GFP-tagged URA3 locus were arrested in metaphase at 30°C using nocodazole, and scored for one GFP focus (maintenance of cohesion) or two foci (loss of cohesion). Over 200 metaphase-arrested cells were counted, and an average was calculated from two or three independent experiments; error bars represent SEM.