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. Author manuscript; available in PMC: 2012 May 6.
Published in final edited form as: Mol Cell. 2011 May 6;42(3):378–389. doi: 10.1016/j.molcel.2011.03.023

Figure 2. Phosphorylation inhibits the cohesion establishment function of Eco1.

Figure 2

(A) Cells of the indicated genotype were grown asynchronously at room temperature and examined by immunofluorescence microscopy, staining for tubulin and DAPI. Cells were scored for cell cycle stage, defining a metaphase cell as large-budded with a single DNA mass and short bipolar spindles. Over 200 cells were counted in two independent experiments; error bars represent the standard error of the mean (SEM).

(B) Strains containing a GFP-tagged URA3 locus were arrested in metaphase at 30°C using nocodazole, and scored for one GFP focus (maintenance of cohesion) or two foci (loss of cohesion). Over 200 metaphase-arrested cells were counted, and an average was calculated from two or three independent experiments; error bars represent SEM.