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. Author manuscript; available in PMC: 2012 Mar 18.
Published in final edited form as: Mol Cell. 2011 Mar 18;41(6):682–692. doi: 10.1016/j.molcel.2011.02.027

Figure 4.

Figure 4

Vectorial folding of NBD1. (A) Schematic of CFP-NBD1 fusion protein showing approximate location of acceptor dye (residue 450) and truncation sites used to generate RNCs. (B) Selected corrected CFP emission spectra for D (black) or DA (red) samples as in (A), truncated at the indicated residues. (C) EFRET between CFP and residue 450 plotted as a function of nascent chain length (n = 3, +/− s.e.m. where shown, or average of two experiments for truncations 535, 584 and 654). (D) Schematic showing experimentally derived stages of cotranslational NBD1 folding.