Figure 2.

Effects of bergamot extracts on the IL-8 release. IB3-1 cells were treated with 80 ng/ml of TNF-α and increasing amounts (a: IC₂₅; b:IC₅₀; c:IC₇₅) of bergamot extracts. After 24 hours, recovered supernatants were analyzed using the Bio-Plex human cytokine IL-8 single-plex (Bio-Rad). (-): untreated IB3-1 cells; (+): IB3-1 cells treated with TNF-α. Results represent the average ± S.D. of three independent experiments. For each experiment duplicate determinations were performed. (*) p < 0.05.