Table 7.
Levels of proteins involved in adipose tissue lipid uptake and mobilization
| Male | Female | ||||
|---|---|---|---|---|---|
| Control | HF | Control | HF | ANOVA | |
| Gonadal fat | |||||
| CPT1 | 100 ± 18 | 55.2 ± 2.1a | 83.4 ± 8.0 | 64.6 ± 12 | D |
| LPL | 100 ± 8 | 89.8 ± 13.8 | 71.4 ± 10.6 | 84.4 ± 12.1 | NS |
| HSL | 100 ± 14 | 58.4 ± 11.0 | 135 ± 17 | 154 ± 32b | S |
| PKA | 100 ± 8 | 131 ± 17 | 90.9 ± 8.2 | 131 ± 16a | D |
| α2-AR | 100 ± 7 | 103 ± 4 | 132 ± 7b | 99.8 ± 12.8a | SxD |
| β3-AR | 100 ± 12 | 127 ± 8a | 137 ± 9b | 155 ± 5b | S, D |
| α2/β3- AR | 1.05 ± 0.11 | 0.82 ± 0.05a | 0.97 ± 0.06 | 0.64 ± 0.06a | D |
| Inguinal fat | |||||
| CPT1 | 100 ± 23 | 34.8 ± 7.6a | 39.6 ± 11.4b | 24.2 ± 7.6 | S, D |
| LPL | 100 ± 23 | 344 ± 35a | 204 ± 36b | 133 ± 36b | D, SxD |
| HSL | 100 ± 37 | 88.8 ± 32.0 | 97.0 ± 15.4 | 98.7 ± 37.6 | NS |
| PKA | 100 ± 27 | 100 ± 12 | 105 ± 16 | 97 ± 18 | NS |
| α2-AR | 100 ± 4 | 89.0 ± 3.1 | 72.9 ± 7.7b | 75.6 ± 10.3 | S |
| β3-AR | 100 ± 10 | 94.5 ± 5.7 | 110 ± 9 | 84.3 ± 5.5 | NS |
| α2/β3- AR | 1.03 ± 0.08 | 0.98 ± 0.07 | 0.81 ± 0.15 | 0.91 ± 0.11 | NS |
Values are expressed in arbitrary units (AU). Mean values of control male rat were set as 100%. Values are expressed as the mean ± S.E.M. of 7 animals per group. ANOVA (p < 0.05): S, sex effect; D, diet effect; SxD, interactive effect; NS, non significant; Student's t-test (p < 0.05): aHF vs Control, bFemale vs Male.