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. 2011 May 16;6(5):e18609. doi: 10.1371/journal.pone.0018609

Figure 6. Nascent strand abundance of the mini-EBV episomes in HCT116 cells.

Figure 6

A view of the mini-EBV genomes is shown on top. The table summarizes the x-folds of enrichment for the nascent strand abundance using fragment J as reference. Fragment J, which does not bind components of the replication initiation machinery [15], is located in the prokaryotic backbone of the mini-EBV episomes as shown in the schematic view of the mini-EBV genome together with the location of the different amplified regions. The enrichment and standard deviation of at least three independent experiments is shown. (Top lines) Nascent strand DNA of p2908 (wild-type oriP) and ΔDS(p2910) were determined in HCT116 cells after two weeks of selection. Both genomes are episomally stably maintained and replicate in an ORC-dependent manner. (Lower lines) Nascent strand abundance of HCT116 cells transiently transfected with the mini-EBV episomes p2908 (wild-type oriP), iFR(p2912) and iDS(p2913). Nascent strand abundance was analyzed 96 h post transfection and without supplying selective pressure. The nascent strand abundance of each PCR-fragment was normalized in relation to the non-origin PCR-fragment J.