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Internalization rates of Nef loop mutant proteins from SIVmac239. Shown is a kinetic internalization assay in 293-T cells. Internalization rates of the Nef DE184AA, LM194AA, DD204AA, and DE184AA/LM194AA/DD204AA mutant proteins were compared with the wild-type Nef protein and the truncated CD8 protein (=negative). Error bars indicate SEs of the mean from three independent experiments.
