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MCF-7 and HT-29 were treated with 50 µM cisplatin for the indicated incubation times (6 h, 10 h, 12 h, and 24 h). As positive control, cells were treated with 500 µM tBHP (tert-butyl hydroperoxide) for 3 h. No significant induction of intracellular ROS with 50 µM cisplatin was observed at the analyzed time points.
