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. 2011 May 16;6(5):e19714. doi: 10.1371/journal.pone.0019714

Figure 8. Changes in gene expression of key regulators at the time of glycolysis and impedance change in MCF7 cells.

Figure 8

To confirm large-scale gene expression profiling data, selected genes with a focus on p53 signalling were analyzed by quantitative real-time RT-PCR using independent samples. A–B, real-time RT-PCR shows strong induction of GADD45A, PMAIP1 and ATF3 (A) and low, but significant regulation of MALAT and SESN1 (up) and CDK6 and E2F3 (down) (B) Control: samples from incubation on biosensor chip without cisplatin treatment; Glycolysis: samples from biosensor chip at time of glycolysis change (Fig. 1D ); Impedance: samples from biosensor chip at time of impedance change (Fig. 1E ). Footnote to Fig. 8. Significance values: * p<0.05; ** p<0.01; *** p<0.001. C, results from gene expression profiling for the same genes. ‡E2F1 is included because it shows similar significance in micro array data as E2F3. Fold change relative to control sample for glycolysis and impedance change. Impedance/Glycolysis: relative ratio of changes in glycolysis and impedance.