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Products of proteolytic digestion of AF1 resolved on an SDS–PAGE gel after treatment with trypsin (Left Panel), Endo Gluc-C (Middle Panel), and chymotrypsin (Right Panel) in absence (lane 2) or presence of increasing concentrations of trehalose (0.2, 0.4, 0.6, 0.8, 1.0, 1.2 M, lanes 3–8, respectively). MW = Molecular Weight Markers; and lane 1 = undigested AF1.
