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. 2011 May 16;6(5):e19689. doi: 10.1371/journal.pone.0019689

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Khan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Gel mobility shift assay showing binding of GR500 with GRE. Left Panel, a native gel showing bands of GR500:GRE complex; and Right panel, data from densitometric analysis of band of complex plotted against increasing concentrations of trehalose. (B) Immuno-reaction to anti-AF1 antibody after immunoprecipitation (IP) from HeLa nuclear extracts (NE) with SRC-1 antibody (Upper Panel). Lane 1, AF1 + beads (negative control). Lane 2, AF1+NE+beads in absence of trehalose. Lane 3, AF1+NE+beads in presence of trehalose. An average densitometric analyses of band intensity showing relative fold induction (Lower Panel).