Skip to main content
. 2011 May 16;6(5):e19523. doi: 10.1371/journal.pone.0019523

Figure 5. Bioinformatics prediction and Northern blot detection of new C. sativus miRNAs.

Figure 5

A) Primary transcripts containing the predicted stem-loop structures precursor of new cucumber miRNAs. The mature miRNAs identified in cucumber sRNAs library are highlighted (green for miRNAs and blue for miRNAs*). The size of the precursors may be slightly longer than represented. B) Low molecular-weight enriched-RNA was extracted from cucumber leaves, electrophoresed and transferred to nylon membranes. The novel cucumber miRNAs were detected by Northern blot assays using non isotopic probes. I and II indicate plants maintained at 25°C and 30°C, respectively, resembling the plant growing conditions used for sRNAs library generation.