Table 1. Known micro RNAs (miRNAs) recovered from our C. sativus sRNA library.
miRNA family | Size range | 5′ end | miRNA* | Homology by specie | |||||
A.thaliana | O.sativa | P.trichocharpa | R.comunis | M.truncatula | V.vinifera | ||||
Conserved | |||||||||
miR156 | 20–23 | U (68%) | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
miR159 | 19–23 | U (77%) | No | Yes | Yes | Yes | Yes | Yes | Yes |
miR160 | 20–21 | U (100%) | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
miR164 | 20–21 | U (67%) | No | Yes | Yes | Yes | Yes | Yes | Yes |
miR165 | 21 | U (100%) | No | Yes | Yes | No | No | No | No |
miR166 | 21–22 | U (100%) | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
miR167 | 21–23 | U (87%) | No | Yes | Yes | Yes | Yes | Yes | Yes |
miR168 | 21–22 | U (75%) | Yes | Yes | No | Yes | Yes | Yes | Yes |
miR169 | 21–23 | U (75%) | Yes | Yes | No | Yes | No | No | Yes |
miR171 | 19–23 | U (65%) | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
miR172 | 19–21 | A/G (50%) | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
miR319 | 20–21 | U (100%) | No | Yes | No | Yes | Yes | Yes | Yes |
miR390 | 19–21 | A (75%) | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
miR393 | 21–23 | U (100%) | No | Yes | Yes | Yes | Yes | Yes | Yes |
miR396 | 21–22 | U (100%) | Yes | Yes | Yes | Yes | Yes | Yes | Yes |
miR397 | 21 | C (50%) | No | Yes | Yes | Yes | Yes | No | Yes |
miR398 | 20–21 | U (100%) | No | Yes | Yes | Yes | Yes | Yes | Yes |
miR399 | 21 | U (100%) | No | Yes | No | No | No | No | No |
miR408 | 21–22 | U (66%) | No | Yes | Yes | Yes | Yes | Yes | Yes |
Non-conserved | |||||||||
miR170 | 21–22 | U (100%) | No | No | No | No | No | No | Yes |
miR827 | 22 | U (100%) | No | No | No | Yes | No | No | No |
miR858 | 22 | U (100%) | No | No | No | Yes | Yes | No | No |
miR894 | 20–21 | G/C(50%) | No | No | No | Yes | No | No | No |
miR1030 | 19 | C (100%) | No | No | No | Yes | No | No | No |
miR2950 | 21–22 | U (100%) | Yes | No | No | No | No | No | Yes |
*The column shows the reads encompassing the defined miRNA sequence ±2 nts.