Table 1. Primers used for the characterization of Psy1 gene in H. chilense.
| Primer | Purpose | Sequence 5′→3′ |
| RvPsy1con1 | 5′ SP | GCCCTCTTGATCTGCCTCTTCATG |
| RvPsy1con3 | 5′ SP | CATATGGCGCGCCGCCGCTCCTC |
| NP for RvPsy1con1 | ||
| RvPsy1con2 | NP for RvPsy1con3 | ACGTCGTACACCTTCTGCTCCGA |
| FwPsy1con2 | 3′ SP | TCGGAGCAGAAGGTGTACGACGT |
| FwPsy1con1 | NP for FwPsy1con2 and FwPsy1con3 | CATGAAGAGGCAGATCAAGAGGGC |
| FwPsy1con3 | 3′ SP | CGGCGCGCCATATGGGCCATCTA |
| FwPsy1Hc2 | 3′ SP | TGTCGGAGAAGATGCAAGAAGAGGA |
| FwPsy1Hc1 | NP for FwPsy1Hch2 | CATCTCAAAGGAGTCGTCACCGAAA |
| HcPsy1-CDS3F | cDNA amplification | CACCACTCCGGCCGAGACAAAG |
| Genomic DNA amplification | ||
| Full-length DNA sequencing | ||
| HcPsy1-CDS4F | cDNA amplificationPhysical mapping | CATAGTGGTGAATCCATCCCTTGC |
| HcPsy1-5ER | Genomic DNA amplification | GTCGTTTGCCTCGATCTCAT |
| HcPsy1-CDS4R | cDNA amplification | CCTGACCATCTTCATCTTGCTTTG |
| HcPsy1-2EF | Genomic DNA amplification | CTTGCTGATGACGGAGGAG |
| Full-length DNA sequencing | ||
| HcPsy1-CDS5R | cDNA amplification | TGTCTTCAAACGCACATGTTCTAGC |
| Genomic DNA amplification | ||
| Full-length DNA sequencing | ||
| HcPsy1-3EF | Full-length DNA sequencing | GCCCCTAGACATGCTCGAA |
| HcPsy1-2ER | Physical mapping | GCCGCTCCTCCGTCATCAG |
| HcPsy1-4EF | Full-length DNA sequencing | CTCGCGAACCAGCTCACC |
| HcPsy1RT-F1 | RT-qPCR | CGAACCAGCTCACCAACATA |
| HcPsy1RT-R1 | RT-qPCR | GAGCTCGTCTTGTGGCAAAT |
SP: specific primer for RACE PCR; NP: nested primer for RACE PCR.