Table 2. Functional analysis of Psy1 transcripts of H. chilense.
Function | |||
Protein type | Transcript tested | Color1 | β-carotene2 |
PSY1Hc-432a | 1431 | Yellow | Presence |
PSY1Hc-432b | 1431 | Yellow | Presence |
PSY1Hc-370a | 1245 | Pale Yellow | Traces |
PSY1Hc-370b | - | - | - |
PSY1Hc-364 | 1227 | Yellow | Presence |
PSY1Hc-342 | 1161 | White | Absence |
PSY1Hc-306 | 1053 | White | Absence |
PSY1Hc-274 | 957 | White | Absence |
PSY1Hc-271 | 1136 | Yellow | Presence |
PSY1Hc-218 | 869 | White | Absence |
PSY1Hc-196 | 712, 741 | White | Absence |
PSY1Hc-175 | 625 | White | Absence |
PSY1Hc-161 | 618 | White | Absence |
PSY1Hc-159 | 612 | White | Absence |
PSY1Hc-86 | 533, 529, 471, 417, 396 | White | Absence |
PSY1Hc-53 | 329, 290 | White | Absence |
Color complementation observed in E. coli colonies co-transformed with pAc-85b plasmid and each of the expression vectors harboring H. chilense Psy1 transcripts.
β-carotene production detected by HPLC in co-transformed E. coli pellets.