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. 2011 May 16;6(5):e19940. doi: 10.1371/journal.pone.0019940

Figure 3. Spectrin cytoskeleton distribution during S. Typhimurium infections.

Figure 3

(a) Immunolocalization of spectrin cytoskeletal elements, spectrin, adducin and p4.1 with actin and DAPI during S. Typhimurium SL1344 invasion in HeLa cells. Actin labeling identified invasion sites with actin-rich membrane ruffles. (b) Images illustrating the inability of a ΔsipA mutant to recruit spectrin to sites of invasion. ΔsipA:sipA restored spectrin recruitment to invasion sites. (c) Spectrin localization to sites of internalized S. Typhimurium SL1344-GFP. Areas of interest are indicated by arrowheads and highlighted in insets. (d) Quantifying invasion efficiency after depletion of spectrin with siRNA as compared to a non-targeting control pool (CP). For each treatment, 3 independent experiments were run; for microscopy counts n = 3, error bars show s.e.m, *P<0.0001 for all statistics. Scale bars are 5 µm.