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. Author manuscript; available in PMC: 2011 Dec 28.
Published in final edited form as: Biochemistry. 2010 Dec 2;49(51):10854–10861. doi: 10.1021/bi101350x

Figure 1.

Figure 1

Effect of amino-terminal deletions on dimerization. (A) Schematic diagram of ABCC1F-FLAG and the NH2-terminally truncated ABCC1281N constructs. The TM segments are shown as boxes. The MRPr1 epitope and the FLAG tag are shown as an oval and circle, respectively. ECL3 and L0 are also indicated. (B) Expression (left) and co-immunoprecipitation (right) of the NH2-terminally truncated ABCC1281N constructs. The ABCC1281N constructs were transiently transfected into HEK293 cells with stable expression of ABCC1F-FLAG. Forty-eight hours following transfection, cells were harvested and lysates were prepared for immunoprecipitation using the anti-FLAG antibody followed by Western blot analysis probed using antibody MRPr1. The smaller band in lanes 3 and 9 is possibly due to degradation.