Skip to main content
. Author manuscript; available in PMC: 2011 Dec 28.
Published in final edited form as: Biochemistry. 2010 Dec 2;49(51):10854–10861. doi: 10.1021/bi101350x

Figure 6.

Figure 6

Effect of ECL3 replacement on dimerization. (A) Schematic diagram of ABCC1F-FLAG and the ABCC1281N constructs with ECL3 replaced by ECL7. The TMs are shown as boxes, and the amino acid sequence of ECL3 or the replacement sequence is shown in single-letter code. The MRPr1 epitope and the FLAG tag are shown as horizontal and vertical ovals, respectively. (B) Expression (left) and co-immunoprecipitation (right) of the wild type and the mutant ABCC1281N constructs. The wild type and the mutant ABCC1281N constructs were transiently transfected into HEK293 cells with stable expression of ABCC1F-FLAG followed by co-immunoprecipitation with the FLAG antibody and Western blot analyses probed with antibody MRPr1. (C) Quantification of the co-immunoprecipitated wild type and the mutant ABCC1281N constructs. The expression and co-immunoprecipitation levels of the wild type and the mutant ABCC1281N constructs in panel B were quantified using ScnImage, and the relative ratios of co-immunoprecipitation level to expression level were calculated.