Skip to main content
. Author manuscript; available in PMC: 2011 Dec 28.
Published in final edited form as: Biochemistry. 2010 Dec 2;49(51):10854–10861. doi: 10.1021/bi101350x

Figure 7.

Figure 7

Mapping the interaction site in the opposing subunit. (A) Schematic diagram of ABCC1155–281N-FLAG and the NH2-terminally truncated ABCC1281N constructs. The MRPr1 epitope and the FLAG tag are shown as an oval and circle, respectively. (B) Co-expression (left) and co-immunoprecipitation (right) of ABCC1155–281N-FLAG with the NH2-terminally truncated ABCC1281N constructs. The NH2-terminally truncated ABCC1281N constructs were transiently cotransfected with ABCC1155–281N-FLAG into HEK293 cells followed by co-immunoprecipitation with the FLAG antibody and Western blot analyses probed with antibody MRPr1 to detect all constructs.