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. Author manuscript; available in PMC: 2012 Jul 1.
Published in final edited form as: Biophys Chem. 2011 Jan 22;156(2-3):115–127. doi: 10.1016/j.bpc.2011.01.006

Figure 5.

Figure 5

a. Temperature effect on the 8-kDa domain - ssDNA interactions in the absence of magnesium. Fluorescence titrations of the ssDNA 10-mer, dεA(pεA)9, with the rat pol β8 -kDa domain (λex = 325 nm, λem = 410 nm) in buffer C (pH 7.0) at different temperatures: (■) 5°C, (❑)10°C, (●),20°C, (❍) 25°C. The concentration of the ssDNA 10 -mer is 1 × 10−6 M. The solid line is the nonlinear least-squares fits of the titration curves, using the single-site binding isotherm defined by eq. 8. b. Fluorescence titrations of the ssDNA 16-mer, dεA(pεA)15, with the rat pol β8 -kDa domain (λex = 325 nm, λem = 410 nm) in buffer C (pH 7.0) at different temperatures: (■) 5°C, (❑)10°C, (●),15°C. (❍) 20°C. The concentration of the ssDNA 10 -mer is 1 × 10−6M. The solid line is the nonlinear lea st-squares fits of the titration curves, using the single-site binding isotherm defined by eq. 8. c. The dependence of the natural logarithm of the macroscopic binding constants, K10 (❑) and K16 (■), upon the reciprocal of the temperature (Kelvin) (van’t Hoff plot). The apparent enthalpies and entropies of the examined binding processes are included in Table 1.