Table 1.
Thermodynamic parameters of the binding of the rat pol β 8-kDa domain to the ssDNA oligomers dεA(pεA)9 and dεA(pεA)15, in buffer C (pH 7.0, 10°C), in the absence and presence of 1 mM MgCl2(details in text)*.
| ssDNA Oligomer | MgCl2 | Stoichiometry | Site-size | **∂LogKN/∂Log[H2O] | ***∂LogKN/∂Log[H2O] | ΔHo kcal/mol | ****ΔSo cal/(mol deg) |
|---|---|---|---|---|---|---|---|
| dεA(pεA)9 | - | 1 | 13 ± 1 | −14.9 ± 2.3 | −14.2 ± 2.3 | −2.8 ± 0.9 | 15 ± 5.0 |
| 1 mM | 1 | 9 ± 1 | −19.1 ± 2.5 | −18.3 ± 2.5 | 0.0 ± 0.8 | 22.0 ± 7.0 | |
| dεA(pεA)15 | - | 1 | 13 ± 1 | −15.9 ± 2.3 | −15.4 ± 2.3 | 13.1 ± 2.8 | 73.0 ± 21.0 |
| 1 mM | 1 | 9 ± 1 | −19.4 ± 2.5 | −16.2 ± 2.5 | 3.3 ± 1.1 | 33.0 ± 9.0 |
*
Errors are standard deviations determined using 3 – 4 independent experiments.
**
Determined in the presence of glycerol.
***
Determined in the presence of DMSO.
****
Calculated using the intrinsic binding constant obtained at 10°C.