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. Author manuscript; available in PMC: 2012 Jun 3.
Published in final edited form as: J Mol Biol. 2011 Apr 2;409(2):202–213. doi: 10.1016/j.jmb.2011.03.049

Fig. 2. Sedimentation equilibrium centrifugation of Cp(−10)149.

Fig. 2

The molecular weight of Cp(−10)149d was determined under nondenaturing conditions (50 mM NaHPO4 buffer, pH 7.2). The open circles represent experimental data and the solid line represents a curve fitted using a single-species model. The upper panel shows the residuals (difference between the fitted and experimental values) as a function of radial position. The molecular weight determined (37,500) is close to that predicted (36,000) for a stable dimer. In the example shown, the Cp(−10)149.C48A.C107A mutant was used. Similar results were obtained for the other Cp(−10)149d constructs described including one, for example, with solubility enhancing mutations G123A.R127A.15