Table 1.
Mutations affecting solubility and capsid assembly
| Mutant proteins | Expression in E. coli | Structural Locationb | ||
|---|---|---|---|---|
| Insoluble | Capsid | Solublea | ||
| Cp149 | ++++ | |||
| Cp143 | +++ | + | ||
| Cp141 | ++ | ++ | ||
| Cp140 | ++++ | |||
| Cp139 | ++++ | |||
| Cp149.T142A | ++++ | Ext | ||
| Cp149.S141A | +++ | + | Ext | |
| Cp149.L140A | ++++ | Ext | ||
| Cp149.N136A | ++++ | PR | ||
| Cp149.Y132A | ++++ | PR | ||
| Cp149.R127A | ++++ | 5α | ||
| Cp149.G123A | ++++ | 5α | ||
| Cp149.Y132A. | ++++ | 5α | ||
| G123A.R127A | ||||
| Cp149.R127A.G123A | ++++ | 5α | ||
| Cp149.TI14A | + | +++ | 5α | |
| Cp149.G123A.T114A | ++++ | 5α | ||
| Cp149.C48A.C107A | ++++ | |||
| Cp149.C48A.C61A.C107A | ++++ | |||
| Cp(−10)149 | ++++ | |||
| Cp(−10)149.G123A | ++ | ++ | ||
a
The soluble protein is always dimeric.
b
Structural locations based on the atomic structure17 where Ext refers to the extended region (residues 137–142); PR, the proline rich region (residues 128–136); and 5α, helical region 5 (residues112–127).