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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Biochem J. 2011 Jun 1;436(2):291–303. doi: 10.1042/BJ20101865

Figure 6. Tail-anchor mutants of Sss1p form Sec61 complexes unstable in Triton X-100 when assayed by low percentage sucrose density gradient centrifugation.

Figure 6

Post-nuclear membranes derived from yeast strains expressing Sss1pWT, Sss1pYG, or Sss1pF were solubilized in 1% Triton X-100 and Sec61 complexes were resolved by sucrose gradient centrifugation through a linear 0–15% sucrose gradient. Gradient samples were divided into 13 fractions, acid precipitated, and analyzed by SDS-PAGE and immunoblotting with antibodies to Sec61p, Sss1p, and Sbh1p. The positions of molecular weight standards run in parallel experiments are shown: Cytochrome c, 12 kDa; BSA, 67 kDa.