Alignments of the γ- subunit from Homo sapiens (1), Arabidopsis thaliana (2), Saccharomyces cerevisiae (3), Methanocaldococcus jannaschii (4) and SecE from Escherichia coli K12 (substrain W3110) (5) made with the EMBL-EBI ClustalW2 program. The GY residues mutated to YG (black solid arrows) and the F insertion (grey solid arrow) in Sss1p (3) are indicated. PrlG mutations in SecE (5) are indicated by open arrows. Identical (*), conserved (:), and semi-conserved (.) residues are indicated.