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. Author manuscript; available in PMC: 2012 Jun 11.
Published in final edited form as: Eur J Pharmacol. 2011 Jan 1;660(1):103–110. doi: 10.1016/j.ejphar.2010.11.040

Figure 4.

Figure 4

Melanocyte co-culture p38 and p53 responses to NDP-MSH and ultraviolet radiation. Co-cultures of melanocortin MC1 receptor wild type (WT–QF1193) or homozygous R151C melanocytes (QF1108) and keratinocytes were treated with 20 mJ/cm2 ultraviolet radiation(U), 20 nM NDP -MSH (N) or both (UN) and incubated for 1 hr. Total cell extracts were used for western immuno-blotting. Activated forms of p38 and p53 were detected using antibodies specific for the Thr180/Tyr182 (pp38) and Ser15 (pp53) phosphorylated sites (Cell Signaling). These blots are representative of 2-3 independent experiments.