. Author manuscript; available in PMC: 2012 Jun 3.

Published in final edited form as: J Mol Biol. 2011 Apr 6;409(2):101–112. doi: 10.1016/j.jmb.2011.03.071

Table 6.

Summary of the kinetic parameters for 3×HA-DGKα WT and V656L mutant. Kinetic analysis shows that the V656L mutation of DGKα increases the V_max of the enzyme for SAG and decreases it for SLG, thus introducing the arachidonoyl preference (see also Fig. 3B). Values of V_maxare relative values since the absolute amount of enzyme in the cell preparations is not known. V_max of 3×HA-DGKα V656L is normalized for the amount of protein relatively to WT. Results are presented as the mean ±S.D.

	K_m, mol%	V_max, nmol PA min⁻¹	V_max/K_m, mol%⁻¹sec⁻¹
3×HA-DGKα WT
SAG	2.1 ± 0.4	1.44 ± 0.09	0.70 ± 0.13
SLG	2.8 ± 0.3	1.51 ± 0.08	0.55 ± 0.07

3×HA-DGKα V656L
SAG	1.8 ± 0.3	1.75 ± 0.11	1.00 ± 0.17
SLG	1.5 ± 0.4	1.26 ± 0.12	0.87 ± 0.25