Figure 1.

Clathrin interacts with Apl2p. Native immunoprecipitations were performed from a lysate of strain LSY93-2A by using affinity-purified Clc1p and GST antibodies. Lanes 1 and 4 correspond to 1/20 of the HSP and HSS fractions used, respectively, for the immunoprecipitations. Lanes 2 and 3 correspond to the immunoprecipitates of an HSP fraction by using GST and Clc1p antibodies, respectively. Lanes 5 and 6 correspond to the immunoprecipitates from an HSS fraction by using GST and Clc1p antibodies, respectively. The HSP/HSS fractions and the immunoprecipitates were subjected to SDS-PAGE and transferred to a nitrocellulose filter. The nitrocellulose was cut into strips and immunoblotted with Clc1p, Chc1p, Apl2p, and Apl1p antibodies.