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. 2001 Feb;12(2):475–485. doi: 10.1091/mbc.12.2.475

Figure 2.

Figure 2

Detection of Vps10p-HA by coimmunoprecipitation with clathrin antibodies. Native immunoprecipitations were performed as in Figure 1. (A) Lanes 1–3 correspond to native immunoprecipitation from WT/ODY50. Lanes 4–6 correspond to native immunoprecipitation from chc1ts/ODY129. Native immunoprecipitations for Kex2p-HA enrichment were performed from WT/ODY47 (lane 1–3) and from chc1ts/ODY117 (lane 4–6). Kex2p-HA and Vps10p-HA were detected by using monoclonal 12CA5 anti-HA antibody. GDPase (B) and invertase (C) activities were performed on HSP and immunoprecipitation fractions from WT/EHY191.