Figure 3.

Vps10p is degraded in chc1ts cells. WT/LSY93–2A and chc1ts/EHY202 strains were radiolabeled for 10 min with [³⁵S]methionine and cysteine directly at 24°C (lanes 1–4) or were incubated for 45 min (lanes 5–9) at 37°C before labeling at 37°C. After the indicated chase times, aliquots of cells were removed and subjected to immunoprecipitation with antibodies to Vps10p. Lane 9 corresponds to Vps10p immunoprecipitation after a 90-min chase at 37°C in a pep4 background (WT/EHY191 and chc1ts/GPY409). The PEP4-dependent cleavage product is indicated (∗). Samples were analyzed by SDS-PAGE and autoradiography.