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Vps10p stability and CPY maturation in WT, apl2Δ, clc1Δ, pep12Δ, and vps34Δ strains. WT/LSY93-2A, apl2Δ/GPY1783-10A, clc1Δ/LSY93-5B, pep12Δ/RSY1306, and vps34Δ/ PHY102 strains were radiolabeled for 10 min with [35S]methionine and cysteine directly at 30°C. After a chase for the indicated times (0, 1, and 2 h), aliquots of cells were removed and subjected to immunoprecipitation with antibodies to Vps10p (A) or CPY (B). The PEP4-dependent cleavage product is indicated (∗).
