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Detection of Vps10CtΔp-HA by coimmunoprecipitation with clathrin antibodies. Native immunoprecipitations were performed as in Figure 1. (A) pRS426vps10G1423stop-HA plasmid was introduced into WT (ODY80), apl2Δ (ODY140), and vps10Δ (ODY141) strains. (B) pRS426vps10G1423stop-HA was introduced into WT (ODY143) and chc1ts (ODY144) strains. Vps10CtΔp-HA was detected by using monoclonal 12CA5 anti-HA antibody.
