Table 1.
Yeast strains used in this study
| Strain | Genotype | Source |
|---|---|---|
| EHY191 | MATa pep4::TRP1 TPI::SUC2::HIS ade2-1 his3-11, 15 leu2-3, 112 trpl-Δ1 ura3-1 | E. Harsay, unpublished |
| LSY93-2A | MATa leu2-3, 112 ura3-52 his3-Δ200 trpl-Δ901 suc2-Δ9 CLC1 | Silveira et al., 1990 |
| LSY93-5B | MATa leu2-3, 112 ura3-52 his3-Δ200 trpl-Δ901 suc2-Δ9 clc1::HIS3 | Silveira et al., 1990 |
| EHY202 | MATα chc1-521(ts) ura3-52 | E. Harsay, unpublished |
| GPY409 | MATa leu2-3, 112 ura3-52 his4 and/or his6 trpl-289 chc1-521(ts) sst1-3 pep::LEU2 | Rad et al., 1995 |
| PHY102 | MATa suc2-Δ9 ura3-52 leu2-3 his3-Δ200 trp1Δ911 lys 2-801 vps34Δ::TRP1 | Herman and Emr, 1990 |
| GPY1783-10A | MATα ura3-52 leu2-3 his3-200 trp1-901 lys2-801 suc1-9 GAL mel apl2::TRP1 | Yeung et al., 1999 |
| RSY1306 | MATα ura3-52 leu2,3-113 his3-Δ200 trp1-Δ901 lys2-801 pep12Δ::HIS3 | Becherer et al., 1996 |
| CCV120 | MATα vps45Δ2::HIS3 his3-Δ200 leu2-3, 112 lys2-801 trp1-Δ901 ura3-52 suc2-Δ9 | Burd et al., 1997 |
| EHY62 | MATα sec6-4 vps10Δ::TRP1 his3-Δ200 leu2-3, 112 lys2-801 trp1-1 ura3-52 | E. Harsay, unpublished |
| ODY117 | EHY202::KEX2HA::URA3 | This study |
| ODY47 | LSY93-2A::KEX2HA::URA3 | This study |
| ODY50 | LSY93-2A::PEP1HA::URA3 | This study |
| ODY129 | EHY202::VPS10HA::URA3 | This study |
| ODY56 | LSY93-5B::VPS10 HA::URA3 | This study |
| ODY62 | GPY1783-10A::VPS10 HA::URA3 | This study |
| ODY63 | RSY1306::VPS10 HA::URA3 | This study |
| ODY54 | PHY102::VPS10 HA::URA3 | This study |
| ODY80 | LSY93-2A/pRS426vps10G1423stop-HA | This study |
| ODY112 | CCY120/pRS426vps10G1423stop-HA | This study |
| ODY114 | RSY1306/pRS426vps10G1423stop-HA | This study |
| ODY140 | GPY1783-10A/pRS426vps10G1423stop-HA | This study |
| ODY141 | EHY62/pRS426vps10G1423stop-HA | This study |
| ODY142 | PHY102/pRS426vps10G1423stop-HA | This study |
| ODY143 | EHY191/pRS426vps10G1423stop-HA | This study |
| ODY144 | GPY409/pRS426vps10G1423stop-HA | This study |