Table 2.

Plasmids used in this study

Plasmid	Description	Source
pGEX-2TGSTCLC1	CLC1 (ORF) was fused to GST gene of pGEX-2T vector	This study
pBAD24CLC1HIS6	CLC1 (ORF) containing 6 HIS at the C terminus was cloned into the pBAD24 vector	This study
pRS306KEX2HA	pRS306 containing KEX2 gene with three copies of the HA epitope inserted at the C terminus of the ORF	Nothwehr et al., 1995
pRS306VPS10HA	pRS306 containing VPS10 gene with three copies of the HA epitope inserted at the C terminus of the ORF	This study
pRS426vps10HAG1423stop	pRS426 containing VPS10 gene with three copies of the HA epitope followed by two stop codons inserted at the codon 1423 of the ORF	This study
pGTEP1	pBluescript II SK (+/−) containing three HA copies	RS strain collection